The key difference between cuvette and test tube is that the cuvette has two straight sides whereas the test tube has no straight sides. Further, cuvettes and test tubes have different applications and chemical compositions as well.
Both cuvette and test tube have common use in analytical chemistry as equipments for analysis. Both these are tube-like equipments that are open at one end and sealed at the other end.
CONTENTS
1. Overview and Key Difference
2. What is a Cuvette
3. What is a Test Tube
4. Similarities Between Cuvette and Test Tube
5. Side by Side Comparison – Cuvette vs Test Tube in Tabular Form
6. Summary
What is a Cuvette?
A cuvette is a tube-like equipment with two straight sides and two circular or straight sides. Further, it has one end sealed while the other end is open. The material used to make it is highly transparent and clear. Ex: plastic, glass, fused quartz, etc.
The use of cuvette in spectroscopic analysis is to keep the sample inside a spectrophotometer. Inside the spectrophotometer, a light beam passes through the sample to measure the absorbance of light by the sample. There, the light beam should pass through the cuvette.
Figure 01: A Cuvette made of Fused Quartz
The cross-section of a cuvette looks like a rectangle. Therefore, a cuvette is like a rectangular test tube. When using a cuvette in an analysis, first the cuvette is filled with the sample to about 80% of the volume inside the cuvette. However, this volume will be different from one cuvette to another.
There are several different names used to name a cuvette. Ex: cell, capillary, quartz cell, spectrophotometer cuvette, etc. In a cuvette, there are two sides through which the light beam passes. These are straight sides. The scratches on these sides can cause light scattering, thus, creating errors in the final result. Therefore, handling the cuvettes carefully to avoid any scratches is important. Even fingerprints and water droplets can cause errors. Therefore, this surface has to be cleaned with a clean cloth or gauze.
What is a Test Tube?
A test tube, also known as a culture tube or sample tube, is a tubular equipment made of glass or plastic. Further, the tube has an opening at one end and sealed at the other end. The sealed end is most of the times circular, flat or conical. Therefore, we should use a special type of racks known as test tube racks to keep the test tubes upright.
Figure 02: Test Tubes
The material used to make the test tubes are mostly expansion-resistant such as borosilicate glass. Thus, the tubes can withstand considerable high temperature without any expansion. The circular bottom of the test tube allows to wash them properly and reduce the mass loss when pouring. In biological aspects, culture tube is the name for test tubes because they hold the culturing of all types of organisms such as bacteria and molds.
What are the Similarities Between Cuvette and Test Tube?
- Both have tube-like structures
- The use of both is to hold aqueous sample solutions
What is the Difference Between Cuvette and Test Tube?
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Cuvette vs Test Tube |
|
| A cuvette is a tube-like equipment with two straight sides and two circular or straight sides. | A test tube is a tubular equipment made of glass or plastic. |
| Shape | |
| A cuvette has two straight sides with a rectangular cross-section. | A test tube is tubular with a cylindrical shape, having a circular cross-section. |
| Use | |
| The major use is in spectroscopic analysis. | The major uses are to handle chemicals, heat substances and grow cultures. |
| Materials Used in Making |
|
| Made of clear, transparent materials such as plastic, glass, fused quartz, etc. | Made of plastic or glass (ex: borosilicate glass). |
| Heat Treatment | |
| Cuvettes are not used for heat treatments for samples. | Test tubes are used for heating a sample. |
| Synonyms | |
| Cells, capillaries, quartz cells, etc. | Culture tubes, sample tubes |
Summary – Cuvette vs Test Tube
Cuvettes and test tubes are chemical equipment that hold liquid samples for analysis. The use of cuvettes are in spectroscopic analysis while that of test tubes are in general applications in a laboratory. The key difference between cuvette and test tube is that the cuvette has two straight sides whereas the test tube has no straight sides.