Electrophoresis and chromatography are both laboratory techniques that are used to test a specific sample. When there is the sample to be tested at a large volume that time the chromatography technique is used, and the electrophoresis technique is generally used for the microscopic level of sample size. Both these laboratory techniques are used vastly almost all around the world.
Electrophoresis vs Chromatography
The main difference between electrophoresis and chromatography is that in electrophoresis, the chemical properties of a particular chemical species are put to use, and on the other hand, in the case of chromatography, a partition is the coefficient of a particular species that has been put to use for testing samples in laboratories.
Electrophoresis is a technique used in a laboratory to test samples by way of using the chemical properties of a particular chemical species. In the sample, a person using this technique can observe the motion of the particle which is dispersed. A person can relate the motion of chemical species under the fluid when it exists.
Chromatography is a technique used in a laboratory to test samples by way of using the partition that is the coefficient of a particular species. This technique is used when the sample size is very vast to be tested. The technique of chromatography is also very useful when it comes to separating small components from a mixture.
Comparison Table Between Electrophoresis and Chromatography
| Parameters of Comparison | Electrophoresis | Chromatography |
| Meaning | Electrophoresis is a technique used in a laboratory to test samples using the chemical properties of a particular chemical species. | Chromatography is a technique used in a laboratory to test samples using the partition that is the coefficient of a particular species. |
| Movement of particles | In electrophoresis, the movement of the particles of the sample takes place due to the electric field which is present between the charged particle of one medium to another. | In chromatography, the movement of the particles of the sample takes place due to the partitioning of components between the mobile phase and stationary face. |
| Form of sample | The technique of electrophoresis can be usually carried out on solid as well as liquid samples. | The technique of chromatography can be carried out on liquid, solid as well gaseous samples. |
| Application | The application used in the technique of electrophoresis is by extracting the DNA fragmentation according to its size. | The application used in the technique of chromatography is by processes of human blood. |
| Volume of sample | The electrophoresis technique is generally used for the microscopic level of sample size. | When there is the sample to be tested at a large volume, the chromatography technique is used. |
What is Electrophoresis?
Electrophoresis is a technique used in a laboratory to test samples by way of using the chemical properties of a particular chemical species. In the sample, a person using this technique can observe the motion of the particle which is dispersed. A person can relate the motion of chemical species under the fluid when it exists.
Electrokinetic is another word used for electrophoresis. There are two categories of electrophoresis which are used to test a sample. These two types are decided to depend on the type of ion that is present in the sample to be tested. These two types are known as cataphoresis and anaphoresis.
The cataphoresis is used mainly for the positively charged ions present in the sample, which is to be tested, and on the other hand, the anaphoresis is used mainly for the negatively charged ions present in the sample, which is to be tested.
What is Chromatography?
Chromatography is a technique used in a laboratory to test samples by way of using the partition that is the coefficient of a particular species. This technique is used when the sample size is very vast to be tested. The technique of chromatography is also very useful when it comes to separating small components from a mixture.
When it comes to the processing of human blood, the chromatography technique plays a very important role in it. In this, the chromosome technique is used to separate the different components present in the human blood for therapeutic use. There are two faces in this technique which are the mobile phase and stationary phase.
The first phase that is the mobile face, helps in the analysis of the sample, and on the other hand, the Stationary phase allows in practising the separation of the different components in a sample. This is exactly how the components are separated in chromatography. Partitioning of components between the mobile phase and stationary phase is the theory used while performing this technique.
Main Differences Between Electrophoresis and Chromatography
- Electrophoresis is a technique used in a laboratory to test samples by way of using chemical properties of a particular chemical species, and on the other hand, Chromatography is a technique used in a laboratory to test samples by way of using the partition that is coefficient of a particular species.
- In electrophoresis, the movement of the particles of the sample takes place due to the electric field which is present between the charged particle of one medium to another, and on the other hand, in chromatography, movement of the particles of the sample takes place due to the partitioning of components between the mobile phase and stationary face.
- The technique of electrophoresis can be usually carried out on solid as well as liquid samples, and on the other hand, the technique of chromatography can be carried out on liquid, solid as well gaseous samples.
- The application used in the technique of electrophoresis is by extracting the DNA fragmentation according to its size, and on the other hand, the application used in the technique of chromatography is by processes of human blood.
- When there is the sample to be tested at a large volume at that time, the chromatography technique is used, and on the other hand, the electrophoresis technique is generally used for the microscopic level of sample size.
Conclusion
Electrophoresis and chromatography are both laboratory techniques that are used to test a specific sample. The techniques of electrophoresis and chromatography have revolutionized through these years. This helps in carrying out investigations in a smooth way in laboratories. When there is the sample to be tested at a large volume that time the chromatography technique is used, and the electrophoresis technique is generally used for the microscopic level of sample size.
Both these laboratory techniques are used vastly almost all around the world. Both these techniques play an important role when it comes to studying DNA and its properties related to them. Both these techniques have their characteristics and features which set them apart.
References
- https://www.sciencedirect.com/science/article/pii/0165993689850228
- https://onlinelibrary.wiley.com/doi/abs/10.1002/jssc.201400587