Electron microscopy has been of a multi-facet use in this technologically driven era. They have made the process of image processing far easier. Tem and Sem are two different types of electron microscopy techniques in use today. It may be difficult to find out the differences between the two. However, the two are considerably different.
Tem vs Sem
The main difference between Tem and Sem is that tem can scan only a limited range of specimens. On the other hand, sem can scan a wide range of samples. Tem enables users to observe the internal details of a sample. In contrast, sem is a convenient option to scan the surface details of a specimen.
Tem refers to a technique wherein a specimen transfers a beam of electrons to create an image. There are several modes of operation in Tem. Some of these are scanning TEM images, conventional imaging, spectroscopy, diffraction, and a combination of those. In addition, it is possible to further enhance the potential of tem by a series of stages and detectors.
Sem refers to a technique wherein an image is produced by making scans using a focused beam of electrons. The pattern to scan the electron beam is a raster scan. Sem allows an individual to see the surface of any material ranging from biological samples to geological specimens. Moreover, sem may have artificial colouration to give off an aesthetic effect.
Comparison Table Between Tem and Sem
| Parameters of Comparison | Tem | Sem |
| Full Form | Tem stands for Transmission Electron Microscopy. | Sem stands for Scanning Electron Microscope. |
| Founder | The credits of the first TEM go to Max Knoll and Ernst Ruska in 1931. | The credits of early scanning microscopy goes to McMullan. |
| Applications | TEM has a practical approach in the field of chemical, physical, and biological sciences. | Sem allows an individual to see the surface of any material ranging from biological samples to geological specimens. |
| Specifications | Tem enables users to observe internal details of a sample. | Sem is a convenient option to scan the surface details of a specimen. |
| Sample Range | Tem can scan only a limited range of specimens. | Sem can scan a wide range of samples. |
What is Tem?
Tem stands for Transmission Electron Microscopy. In this microscopy technique, a specimen transfers a beam of electrons to create an image. Transmission Electron Microscopes are significantly superior to light microscopes as they can image at a comparatively higher resolution. As a result, the device can take into account every minute detail of an item.
TEM has a practical approach in the field of chemical, physical, and biological sciences. It is a technique of extensive use in the fields of virology, material sciences, and cancer research, nanotechnology, pollution, palynology, palaeontology, and semiconductor research. Thus, TEM has multiple uses in the modern world.
There are several modes of operation in Tem. Some of these are scanning TEM images, conventional imaging, spectroscopy, diffraction, and a combination of those. Observed closely, any TEM image is a collection of poliovirus. The credits of the first TEM go to Max Knoll and Ernst Ruska in 1931. TEM is also regarded as an essential item in the field of nanoscience.
A tem consists of a vacuum system, specimen stage, electron gun, electron gun, and apertures. Moreover, there are several imaging methods in tem. It is possible to further enhance the potential of tem by a series of stages and detectors. To conclude, tem has become a technique of common use in the present.
What is Sem?
Sem stands for Scanning Electron Microscope. In this technique, an image is produced by making scans using a focused beam of electrons. The pattern to scan the electron beam is raster scan. There are a few sems that have the potential to achieve a resolution that is way better than 1 nanometer.
In a Sem, the sample observation takes place in a high vacuum or a low vacuum or wet conditions. The credits of early scanning microscopy go to McMullan. The image production in a sem is an outcome of the interaction of the electron beam with atoms. Various types of signals come into being in the sem.
Sem allows an individual to see the surface of any material ranging from biological samples to geological specimens. Sem is a fast scanner that provides accurate details. It also enables an individual to make observations with little or no sample preparation. Although sem doesn’t provide 3D images, it allows a user to obtain 3D data using several methods.
Sem has been used to measure the roughness of ice crystals. Some other practical applications of sem are in examining fracture surface of metals, corrosion measurement, fractal dimension, and dimensional measurements. Sem may have artificial colouration to give off an aesthetic effect. To conclude, Sem has a wide variety of uses in the practical arena.
Main Differences Between Tem and Sem
- Tem stands for Transmission Electron Microscopy. In contrast, Sem stands for Scanning Electron Microscope.
- Tem can scan only a limited range of specimens. On the other hand, sem can scan a wide range of samples.
- The credits of the first TEM go to Max Knoll and Ernst Ruska in 1931. In contrast, the credits of early scanning microscopy go to McMullan.
- Tem enables users to observe the internal details of a sample. On the other hand, sem is a convenient option to scan the surface details of a specimen.
- TEM has a practical approach in the field of chemical, physical, and biological sciences. In contrast, Sem allows an individual to see the surface of any material ranging from biological samples to geological specimens.
Conclusion
To sum up, tem and stem vary on several grounds. Tem and sem have different full forms, applications, meanings, and efficiency rates. Tem stands for Transmission Electron Microscopy. TEM has a practical approach in the field of chemical, physical, and biological sciences. On the other hand, Sem stands for Scanning Electron Microscope.
Transmission Electron Microscopes are significantly superior to light microscopes as they can image at a comparatively higher resolution. Sem, on the other hand, also enables an individual to make observations with little or no sample preparation. Although sem doesn’t provide 3D images, it allows users to obtain 3D data using several methods. Conclusively, both tem and sem have multiple uses.
References
- https://www.sciencedirect.com/science/article/pii/S0091679X04740170
- https://www.sciencedirect.com/science/article/pii/S0166516218311571